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. Author manuscript; available in PMC: 2024 Dec 10.
Published in final edited form as: Microbes Infect. 2006 Apr 21;8(7):1732–40. doi: 10.1016/j.micinf.2006.02.009

Fig. 6.

Fig. 6

TLR-2 and MD-2/TLR-4 are not required for TsAg-induced CXCL8 promoter activity. HeLa cells were grown to approximately 80% confluency and then co-transfected with plasmids containing CXCL8 promoter-linked to a Firefly luciferase reporter and either (A) TLR-2 or (B) MD-2 constructs together with a control plasmid, pTK-rLUC, which constitutively expresses low levels of Renilla luciferase. Cells were stimulated the next day with either TsAg (100 μg/ml), peptidoglycan (PG, 1 μg/ml) or LPS (1 μg/ml) and lysates assayed for luciferase activity. Data shown are CXCL8 promoter activity after normalisation of Firefly luciferase activity for control Renilla luciferase activity. Results are the mean ± SEM of a triplicate experiment. Open bars are data from cells transfected with a vector control construct, black bars represent cells transfected with the TLR-2 construct, and hatched bars represent cells transfected with the MD-2 construct.