Extended Data Figure 11. Combination therapies in melanoma, lung, pancreatic and colorectal murine cancer models.

a, Scheme outlining the re-injection of YUMM1.7^OVA R^TT cells into tumor-free responder mice from Fig. 5e (left) and tumor growth of re-challenged mice (n=4 mice for COX2i + Ftl3L, n=3 COX2i and n=2 COX2i+ 5-AZA) or naive control mice (n=5 mice). b, Scheme outlining the injection of YUMM3.3 R^TT tumors into C57BL/6 mice and the treatment regimen with COX2i, Flt3L, 5-AZA and anti-PD1/CTLA-4 (ICB) or isotype control. c, Response to control vehicle treatment or vehicle + ICB treatment of YUMM3.3 R^TT tumors (n=6 mice). d, Response to single treatments and ICB combination treatments of YUMM3.3 R^TT tumors. COX2i, Flt3L, 5-AZA+ICB, COX2i+ICB and Flt3L+ICB (n=8 mice), COX2i+5-AZA+ICB and COX2i+Flt3L+ICB (n=10 mice). e, Survival plot. f, Scheme outlining the injection of CT-26 R^TT tumors into BALB/c mice and the treatment regimen with COX2i, Flt3L, 5-AZA and anti-PD1 (ICB) or isotype control. g, Response to treatment of CT26 R^TT tumors. Vehicle and Vehicle+ICB (n=8 mice), COX2i+5-AZA+ICB and COX2i+Flt3L+ICB (n=9 mice). h, Survival plot. i, Scheme outlining the intrapancreatic injection of EPP2^Luc cells into C57BL/6 mice and the treatment regimen with COX2i, Flt3L and anti-PD1 (ICB) or isotype control. j, Left, representative BLI images of EPP2^Luc tumor burden and right, quantification of control (n=5 mice), anti-PD1 (n=6 mice), anti-PD1 + COX2i + Ftl3L (n=6 mice). k, Survival plot of j. l, Scheme outlining the injection of KPAR tumors into C57BL/6 mice and the treatment regimen with COX2i, Flt3L and anti-PD1 (ICB) or isotype control. m, Response to treatment of KPAR tumors, vehicle ± ICB (n=6 mice) and ICB + COX2i + Ftl3L (n=8 mice). n, Survival plot of m. o, Scheme summarizing the role of inflammatory monocytes in T cell restimulation and the functional convergence of PGE₂ and IFN-I to determine an inflammatory TME, T cell restimulation and immunotherapy response. Bar graphs depict the mean ± s.e.m. Statistical analysis was performed with a one-way ANOVA with Tukey’s multiple comparisons test in j. Log-rank Mantel Cox test in h, k, n. ns=not significant.