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. Author manuscript; available in PMC: 2025 Jan 7.
Published before final editing as: ACS Catal. 2025 Jan 2:1046–1060. doi: 10.1021/acscatal.4c05944

Figure 3. Spectral changes observed upon aerobic mixing of VAD with dithionite.

Figure 3

(a) Wild-type; (b) P151L; (c) P151G; (d) P151V; (e) (P151I; (f) P151N; (g) P151T. Protein and dithionite concentrations are 30 μM and 1.5 mM, respectively. The spectra measured before and immediately after the addition of dithionite, and after 10-minute incubation are in red, green, and blue, respectively. In all cases, the proteins are re-oxidized after 10 minutes.