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| Substrate | kred (s-1) | Kd (μM) | |
|---|---|---|---|
| Wild type | vanillyl alcohol | 0.50 ± 0.01 | 24.2 ± 3.3 |
| P151L | vanillyl alcohol | 339 ± 11 | 1060 ± 80 |
Reactions were performed anaerobically at 25 °C in 50 mM potassium phosphate pH 7.5, 150 mM NaCl. Data are shown as average values ± standard deviation. n = 3 independent experiments.
