REAGENT or RESOURCE	SOURCE	IDENTIFIER
Experimental models: Drosophila melanogaster strains
w1118	Bloomington Drosophila Stock Center	FlyBase: FBgn0003996
ft-EGFP	Hale et al.30	FlyBase: FBal0385338
ds-EGFP	Brittle et al.16	FlyBase: FBal0344517
ft1	Bloomington Drosophila Stock Center	FlyBase: FBst0000304
ftG-rv	Bloomington Drosophila Stock Center	FlyBase: FBst0001894
ds1	Clark et al.31	FlyBase: FBal0003119
dsUA071	Adler et al.32	FlyBase: FBal0089339
dGC13	Mao et al.11	FlyBase: FBal0128007
Df(2L)BSC201	Bloomington Drosophila Stock Center	FlyBase: FBab0044944
sqh∷sqh-3xmKate2	Pinheiro et al.17	FlyBase: FBal0358816
nub-GAL4	Bloomington Drosophila Stock Center	FlyBase: FBtp0009119
dpyRNAi (GD4443)	Vienna Drosophila RNAi Center	FlyBase: FBal0209383
Software
NIS Elements AR version 4.60	Nikon	N/A
FIJI version 2.9.0	https://fiji.sc 33	PMCID: PMC3855844
Tissue Analyzer version	https://grr.gred-clermont.fr/labmirouse/software/WedPA/ 34	PMID: 27730585
RStudio version 3.6.1	http://www.rstudio.com/	N/A
QuantifyPolarity version 2.0	Tan et al.35	PMCID: PMC8451067
Reagents
Heptane glue	Heptane glue was made by placing Sellotape in heptane.	N/A
Gary’s Magic Mountant (GMM)	50% Canada balsam, 50% methylsalicylate	N/A