Fig. 6. Fluorescence microscopy analysis of hMSC response to 2D nanostructured titanium surfaces after 24 h.
(A) Representative LIVE/DEAD stained images showing viable hMSCs on flat, nanospike (NS), and nanonetwork (NN) surfaces. Scale bar is 50 μm. Quantification of (B) hMSC cell numbers, (C) cell area and (D) cell perimeter was performed using Image J software. A threshold was applied, and then particle analysis was performed on the images.
