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. Author manuscript; available in PMC: 2025 Apr 2.
Published in final edited form as: Biomater Adv. 2024 Jan 12;158:213766. doi: 10.1016/j.bioadv.2024.213766

Fig. 9. Image quantification of hMSCs following 24 h co-culture on 2D cpTi surfaces.

Fig. 9

hMSCs were incubated for 24 h on flat control, NS or NN 2D cpTi surfaces, followed by incubation for a further 24 h with S. aureus (A-C) or P. aeruginosa (D–F). hMSCs were stained with LIVE/DEAD and imaged using a fluorescence microscope. Cell number (A,D), area (B,E), and perimeter (C,F) were quantified using Image J software. A threshold was applied, and then particle analysis was performed on the images. * P < 0.05, **** P < 0.001.