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Composition of mobile phase solvents for HPLC-based separation
| Mobile phase solvents for positive polarity MS mode: | |
|---|---|
| Components | Final concentrations |
| Solvent A | |
| Water | 99.90% |
| Formic acid | 0.10% |
| Solvent B | |
| Methanol | 99.90% |
| Formic acid | 0.10% |
| Mobile phase solvents for negative polarity MS mode: | |
|---|---|
| Components | Final concentration |
| Solvent A′ | |
| Water | |
| Ammonium acetate | 5 mM |
| Solvent B′ | |
| Acetonitrile | 100% |
Note: Use LC-MS grade reagents to make these solvents
