. Author manuscript; available in PMC: 2025 May 28.

Published in final edited form as: Biochim Biophys Acta. 2005 Aug 15;1753(1):51–63. doi: 10.1016/j.bbapap.2005.07.006

Table 1. Some conditions employed in the literature to generate fibrils from full-length β₂m in vitro. SDS is sodium dodecyl sulphate and SAP is serum amyloid P component.

Conditions for extension	Conditions for seed generation	Reference
Acidic pH with varying ionic strength	–	[21,29,30,34,54,74]
Dialysis with pH 7.4 buffer containing 200 μg mL ⁻¹ SAP	–	[78]
Dialysis against deionised water and subsequent evaporation of solvent	–	[79]
Incubation in the supernatants of cultured peripheral blood mononuclear cells	–	[80]
pH 7.4, 150 mM KCl, 1M urea with 200 μM Cu²⁺	–	[81]
pH 7.4	Ex vivo amyloid fibrils	[61,82]
Extension of seeds during refolding experiments, pH 7.4	Ex vivo amyloid fibrils	[82]
Extension of seeds in the presence of ∼0.5 mM SDS pH 7.5	Ex vivo amyloid fibrils extended at low pH in vitro	[83,84]
Extension of seeds in the presence of ∼20% v/v TFE and ∼10−200 μg mL⁻¹ heparin, pH 7.5	Ex vivo amyloid fibrils extended at low pH in vitro	[85]

Table 1. Some conditions employed in the literature to generate fibrils from full-length β2m in vitro. SDS is sodium dodecyl sulphate and SAP is serum amyloid P component.