Figure 1. scRNA atlases of the entire nervous system of three Caenorhabditis nematode species.
(A) Phylogenetic tree of species in the Elegans group, adapted from Stevens et al.27 Scale: substitutions per site.
(B) UMAP representations of sequenced cells colored by tissue in 3 Caenorhabditis species.
(C) UMAPs of all neuronal cells. Neuron identities were determined based on 1:1:1 ortholog genes differentially expressed in C. elegans.
(D) Correlation heatmaps of pseudobulked normalized expression levels of 1,380 differentially expressed 1:1:1 orthologs (marker_score > 0.1 in at least one species and one neuron class). Left: rows and columns are grouped by species and then by neuron class. Right: same data, but rows and columns are arranged based on hierarchical clustering. The triplets of homologous neuron classes clustered together across species. See also Figure S1 and Data S1.