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. Author manuscript; available in PMC: 2026 Jan 8.
Published in final edited form as: Algal Res. 2025 Aug;90:104208. doi: 10.1016/j.algal.2025.104208

Fig. 1. Genetic constructs and expression analysis of cyanobacterial FBP/SBPase in C. reinhardtii.

Fig. 1

(a) Schematic representation of expression cassettes used to generate FBP/SBPase expressing lines both with or without mVenus (YFP) fused at the C-terminus. To drive gene expression the hybrid promoter HSP70-RBCS2 was used, while the FBP/SBPase was targeted into chloroplast by exploiting the PsaD target peptide sequence (Photosystem I Subunit D). Both expression cassettes contained the Aph VIII gene confer resistance to the antibiotic paromomycin. Both FBP/SBPase proteins either fused or not to YFP carried a C-terminal S-tag for immunodetection and protein purification. (b) Immunoblot against S-tag showing the FBP/SBPase accumulation in transformed lines without YFP as reporter protein. (c) Fluorescence screening of transformed lines with FBP/SBPase_YFP. The best five lines were subjected to immunoblot against YFP confirming the presence of the protein are reported in the Panel c insert.