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. Author manuscript; available in PMC: 2026 Jan 8.
Published in final edited form as: Algal Res. 2025 Aug;90:104208. doi: 10.1016/j.algal.2025.104208

Fig. 6. Immunoblot analysis of photosynthetic subunit accumulation.

Fig. 6

(a) Immunoblot analysis performed on UVM4 and FBP/SBPase expressing lines on total protein extract using specific antibodies for PsaA, RuBisCO, ATPase C-subunit (AtpC), PetC, CP43, LHCII, and carbonic anhydrase (CAH3). Different amounts of cells were loaded (3, 5 or 9 *106 cells). Immunoblot analysis using a Histone 3 (H3) specific antibody was performed as loading control. Immunoblot signals reported in panel (a) were analyzed by densitometry to determine the relative protein abundance on cell basis (b), on chlorophyll basis (c) or on H3 basis (d). Each protein level was normalized to the UVM4 protein level. Error bars are reported as standard deviations. Asterisks indicate values that are significantly different compared to the background strain UVM4 (Student’s t-test, P < 0.05).