Skip to main content
. Author manuscript; available in PMC: 2026 Feb 4.
Published in final edited form as: Cell Rep. 2025 Feb 24;44(3):115325. doi: 10.1016/j.celrep.2025.115325

Figure 3. Efficient termination requires exonuclease activity by Rat1 and endonuclease activity of RNAPI.

Figure 3

(A) Schematic of the in vitro termination assay. A description is given in the text. For a detailed version, see Figure S3.

(B) In vitro termination assay. The western blot shows Rpa135-HTP (RNAPI) distribution between the DNA-bound (B) fraction and the supernatant (S) that represents terminated and released RNAPI.

(C) Quantitation of data in (B). Some release of RNAPI from the template DNA was already observed at T0, so this initial level was set to 0. The subsequent increase in RNAPI release over time is indicated by the graphs. Complete release is 100%. See methods for the details. Data are represented as mean ± SD. *p < 0.05, two-tailed t test, n = 5).

(D) Two potential mechanisms participating in torpedo termination of RNAPI: (1) Rat1-mediated mechanism and (2) backtracking-mediated mechanism.