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. Author manuscript; available in PMC: 2026 Jun 27.
Published in final edited form as: Biomaterials. 2020 Feb 13;240:119854. doi: 10.1016/j.biomaterials.2020.119854

Fig. 2. More nuclear folds and improved migration in cells from 3D ESP and Lamin A and C influences F-actin organization.

Fig. 2

a, Migration of hMSCs from 300PEOT55PBT45 2D films or 3D ESP scaffolds through a transwell with 8-μm or 3-μm pores. 8 μm ESP: n = 18; 3 μm ESP n = 19; 8 μm Film n = 18; 3 μm Film n = 19, from 4 independent experiments.Krusal Wallis test; *p < 0.05, ***p < 0.001. b, Representative images of examples of folded and non-folded nuclei of hMSCs grown on 300PEOT55PBT45 2D films, 3D AM or 3D ESP scaffolds, stained with Lamin A and C (green), scalebars 5 μm. Arrowheads indicate what was considered a nuclear fold. The graph shows the corresponding quantification. Total counted nuclei for films: 491, AM: 159, ESP: 79, in 8, 11 and 10 different images, respectively, from 2 independent experiments. Kruskal Wallis test, **p < 0,01, ****p < 0,0001 compared to films. c, hMSCs transduced with scrambled-(SCR, left) or LMNA-shRNA (right), cultured on TCP, stained for F-actin (green) and nuclei (blue). The bottom panels are 7 × magnifications of the respective images above. Scale bars represent 30 μm (top panels) and 5 μm (bottom panels). d, Quantification of the F-actin intensity distribution in hMSCs transduced with scrambled- or LMNA-shRNA, cultured on 2D TCP. n = 33 for SCR and n = 29 for LMNA-shRNA from 3 biological replicates. Two-way ANOVA; ****p < 0.0001. e, Quantification of F-actin intensity that overlaps with nuclei staining in hMSCs transduced with scrambled- or LMNA-shRNA. n = 25 cells analyzed for SCR and n = 22 for LMNA from 3 biological replicates. Mann-Whitney test, ****p < 0.0001. a-e, Error bars represent mean ± 95% CI. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article).