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. 2020 Sep 28;44(6):2527–2536. doi: 10.3892/or.2020.7782

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Copyright: © Ji et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Cranial irradiation inhibits cellular proliferation and neurogenesis in the rat hippocampus. Confocal micrographs of hippocampal sections labeled with BrdU (red), DCX (green), GFAP (green) and NeuN (green). (A and B) Proliferating cells labeled with BrdU. (C and D) Immature neurons labeled with BrdU and DCX. (E and F) Differentiated astrocytes labeled with BrdU and GFAP. (G and H) Recently-divided mature neurons labeled with BrdU and NeuN. All histograms represent the mean ± SEM. *P<0.05 and **P<0.01. BrdU, 5-bromodeoxyuridine; DCX, doublecortin; GFAP, glial fibrillary acidic protein; NeuN, neuronal nuclei. Green: (C) DCX, (E) GFAP, and (G) NeuN; Red: BrdU.