Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Oct 23;44(6):2503–2516. doi: 10.3892/or.2020.7823

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Shi et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 7. — HNF1A-AS1 is downregulated due to promoter hypermethylation. (A) Bioinformatics analysis of CpG island in promoter region of HNF1A-AS1. (B) HNF1A-AS1 expression was increased following 5-Aza-dC treatment at different concentrations for 24, 48 and 72 h in TU-686 and TU-177 cells. (C) Methylation status of BSP for the HNF1A-AS1 CpG island in TU-686 and TU-177 cells treated with or without 5- Aza-dC after 48 h. White circle, unmethylated CpG dinucleotide; black circle, methylated CpG dinucleotide. (D) Methylation status of CpG island in HNF1A-AS1 promoter determined by MSP assay in TU-686 and TU-177 cells treated with or without 5-Aza-dC after 48 h. Data are presented as the mean ± SD. All assays were performed in triplicate, and the values represent the mean of three independent experiments. For statistical analysis, ANOVA with Tukey's post hoc test was used for data in (B and D) **P<0.01, ***P<0.001, ****P<0.0001. M, amplification with methylated primers; U, amplification with unmethylated primers; 5-Aza-dC, 5-Aza-2′-deoxycytidine; BSP, bisulfite genomic sequencing; HNF1A-AS1, hepatic nuclear factor 1 α antisense RNA 1; MSP, methylation-specific polymerase chain reaction.