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. 2020 Oct 5;44(6):2537–2546. doi: 10.3892/or.2020.7790

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Copyright: © Munakarmi et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Effects of PF on the expression of Pin1, cyclin D1, β-catenin and EMT markers in HCC Hep3B and Huh7 cells. (A) Evaluation of Pin1, cyclin D1, β-catenin and EMT-associated protein expression by western blot analysis. (B) The protein levels of MMP-2 and MMP-9 in Hep3B and Huh7 cells after PF treatment was detected by western blot analysis. (C) The detection of pro-MMP-2 and pro-MMP-9 activity in supernatants was determined by gelatin zymography assay. PF, Poncirus fructus; HCC, hepatocellular carcinoma; MMP, matrix metalloproteinase.