Table I.
Gene | Oligonucleotide primers |
---|---|
miR-7 | F: 5′-ACGTTGGAAGACTAGTGATTT-3′ |
R: 5′-TATGGTTGTTCTGCTCTCTGTCTC-3′ | |
U6 | F: 5′-ATTGGAACGATACAGAGAAGATT-3′ |
R: 5′-GGAACGCTTCACGAATTTG-3′ | |
SP1 | F: 5′-GACTAGGGCTGCAAGTAGTGAGGA-3′ |
R: 5′-GGAAACTGGAGCACTGGGTAGAC-3′ | |
TP53BP1 | F: 5′-AGCAGGAGCTGGCTATATCCTTGA-3′ |
R: 5′-GACAATGCTGATCCGCAATTAGAA-3′ | |
GAPDH | F: 5′-TCCTGTGGCATCCACGAAACT-3′ |
R: 5′-GAAGCATTTGCGGTGGACGAT-3′ |
miR-7, microRNA-7; SP1, specific protein 1; TP53BP1, tumor suppressor p53-binding protein 1; F, forward; R, reverse.