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. 2020 Nov 4;16:421. doi: 10.1186/s12917-020-02616-9

Fig. 2.

Fig. 2

Agarose gel electrophoresis for amplicons of direct PCR assays for testing dermatophytes DNA from calves’ hair samples. (a) Pan-dermatophytes PCR amplicons of pchs-1gene at 440 bp, (b) One-step PCR ITS+ amplified products for T. verrucosum at 900 bp (lanes 2–8) and T. mentagrophytes at 872 bp (lanes 11, 12), and (c) ITS-1 amplicons of nested PCR at 400 bp. Lanes M: 100 bp molecular size marker, lane Pos.: positive control and lane Neg.: negative control