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. 2020 Nov 4;20:507. doi: 10.1186/s12870-020-02724-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — sl-MH-1 mutant displays increased resistance to P. oryzae. a 3-week-old MH86 and sl-MH-1 seedlings were inoculated with P. oryzae conidia by spraying. At 7 days post inoculation (dpi), the diseased leaves were imaged. b Punch inoculation with P. oryzae conidia was carried out on the leaves of 4-week-old MH86 and sl-MH-1 plants. The infected leaves were photographed at 9 dpi (left); meanwhile fungal biomass was measured to quantify the relative P. oryzae growth in MH86 and sl-MH-1 leaves (right). Bars represent mean values ± SD from three biological replicates. Statistically significant difference was indicated by ** (p < 0.01, Student’s t-test). c. Leaf sheathes of MH86 and sl-MH-1 were inoculated with P. oryzae conidia to monitor the infection process. The infected cells were imaged at 48 and 72 hpi with a microscope under bright field. Bar = 20 μm