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. 2020 Nov 3;11:465. doi: 10.1186/s13287-020-01989-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Sufu positively regulates TNFα-induced inflammatory gene expression in AdSPC-derived iSMCs. a–d Inflammatory gene expression in iSMCs was positively modulated by Sufu. AdSPCs were infected with non-target (sh-NT) or Sufu (sh-Sufu) shRNA lentivirus (a, c), or control (pCDH) or Sufu over-expressing (pCDH-Sufu) vector (b, d), followed by iSMC differentiation for 4 days. Total RNAs and conditioned culture medium were harvested and subjected to RT-PCR (a, b) and ELISA (c, d) analyses, respectively. e, f Luciferase activity assays. AdSPCs infected with sh-NT or sh-Sufu (e), or pCDH or pCDH-Sufu (f) were transfected with IL-6 (pmIL-6 FL) or MCP-1/CCL2 (pMCP-Luc) gene promoter reporters as indicated in the figures, followed by iSMC differentiation. Two days later, total cell lysates were harvested and subjected to luciferase activity assays. The data presented here are mean ± S.E.M. of five (n = 5 in a, b, e and f) or six (n = 6 in c and d) independent experiments. *P < 0.05 (vs sh-NT or pCDH, unpaired t test)