Fig. 6.

Transplantation of AdSPCs increases adversely arterial remodelling in response to injury, while miR-214-3p over-expression in AdSPCs reverses such processes. Eight-week-old male mice were randomly subjected to arterial injury and AdSPC transplantation. After injury, 100 μl of Geltrex contained culture medium (vehicle ctrl) or 1 × 10⁶ AdSPCs transfected with control AgomiR (AdSPCs/Cel-miR-67) or miR-214-3p AgomiR (AdSPCs/miR-214) was immediately applied and packed around the injured vessel. One (a) or 4 (b, c) weeks later, injured segments of the femoral arteries were harvested and subjected to the following studies as indicated. a RT-qPCR detection of gene expressions in the injured arteries. The data presented in a are mean ± S.E.M. of five independent experiments (femoral arteries from 3~5 mice were pooled for each experiment, n = 5 experiments). b, c Morphometric analysis of the wire injury-induced neointima formation. Paraffin sections from the indicated groups (n = 10 mice for each group) were prepared and subjected to H&E staining analyses. Representative images (b) and morphological characteristics including the neointimal area, neointimal/media (N/M) ratio and media area (c) were presented here. *P < 0.05 (vs vehicle Ctrl). ^#P < 0.05 (AdSPCs/miR-214 vs AdSPCs/Cel-miR-67) (one-way ANOVA with a post hoc test of Tukey’s analysis)