Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 3;20:535. doi: 10.1186/s12935-020-01592-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 3 — DARS-AS1 modulates JAG1 to activate Notch pathway by binding with miR-628-5p. a ENCORI predicted 5 potential target genes of miR-628-5p. b RT-qPCR unveiled that only the expression levels of JAG1 was markedly reduced in CC cells transfected with miR-628-5p mimics. c The binding sites between miR-628-5p-WT and JAG1-WT were exhibited by ENCORI, and the sequences of miR-628-5p-Mut and JAG1-Mut were designed. d RBP-JK luciferase reporter assay and western blot analysis confirmed the inactivation of DARS-AS1 depletion on Notch pathway. e RIP assay followed by RT-qPCR confirmed the strong enrichment of DARS-AS1, miR-628-5p and JAG1 in Ago2-involved RISC in CC cells. f RNA pull down assay disclosed the high enrichment of DARS-AS1 and JAG1 in Bio-miR-628-5p-WT groups in CC cells. g The combination between JAG1 and miR-628-5p at predicted binding sites was verified via luciferase reporter assay. h Luciferase reporter assay determined that DARS-AS1 competed with JAG1 to interact with miR-628-5p in CC cells. All data were displayed as the mean ± SD from three independent experiments. **P < 0.01, n.s.: no significance