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. 2020 Nov 4;18:176. doi: 10.1186/s12964-020-00664-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Clathrin and dynamin deficiency reduces activation of the non-canonical NF-κB pathway. a-c A549, HEK293T and CCD1070Sk cells were transfected with siRNAs targeting clathrin (CHC) (two oligonucleotides) along with control, non-targeting siRNAs (two oligonucleotides) and stimulated or not with Ago for 1 h. d, f A549 cells were transfected with siRNAs targeting dynamin-1/2 (three combinations of oligonucleotides targeting dynamin-1 and dynamin-2, see Methods) along with non-targeting control (Ctrl) siRNAs (two combinations of oligonucleotides, see Methods) and stimulated or not with Ago for 1 h. e, g A549 cells were treated with dynasore (DYN) or DMSO and stimulated or not with Ago for 1 h. Lysates of cells were analyzed by Western blotting with antibodies against the indicated proteins. Representative blots are shown. Values presented below blots represent the averaged p52/p100/loading control (a-e) or NIK/loading control ratio (f-g) from at least three experiments (normalized to the selected control, set as 1) in cells stimulated with Ago