Table 3.
DNA methylation (LINE-1 and MGMT) and histone modification (H3K36me3) of all subjects stratified by quartiles of urinary 1-OHP concentration
| Epigenetic modifications | Urinary 1-OHP concentration (μg g−1 creatinine) | P-trenda | P-trendb | P-trendc | |||
|---|---|---|---|---|---|---|---|
| Q1 (n = 66) | Q2 (n = 68) | Q3 (n = 66) | Q4 (n = 67) | ||||
| LINE-1 (% 5mC) | |||||||
| Mean (95% CI) | 83.65 (82.97, 84.33) | 82.80 (82.24, 83.35) | 81.74 (81.13, 82.35) | 81.72 (81.09, 82.34) | <0.001 | <0.001 | <0.001 |
| Median (IQR) | 84.06 (81.69–85.81) | 82.61 (81.07–84.49) | 81.39 (79.97–83.00) | 81.59 (80.46–83.19) | |||
| MGMT (% 5mC) | |||||||
| Mean (95% CI) | 6.14 (5.06, 7.22) | 4.55 (3.82, 5.27) | 3.85 (3.49, 4.22) | 3.69 (3.25, 4.13) | <0.001 | <0.001 | <0.001 |
| Median (IQR) | 5.11 (3.63–7.13) | 3.48 (2.17–5.09) | 3.42 (2.83–4.60) | 3.19 (2.71–4.21) | |||
| H3K36me3 (relative %) | |||||||
| Mean (95% CI) | 9.32 (7.96, 10.68) | 12.36 (8.28, 16.44) | 17.68 (13.58, 21.78) | 14.99 (11.96, 16.02) | <0.001 | <0.001 | <0.001 |
| Median (IQR) | 8.44 (4.11–12.94) | 8.49 (5.05–13.40) | 13.15 (8.44–19.97) | 11.93 (7.99–18.77) | |||
1-OHP, 1-hydroxypyrene; 5mC, 5-methylcytosine; IQR: interquartile ranges; 95% CI: 95% confidence intervals.
aCrude.
bAdjusted for grilled food consumption.
cAdjusted for grilled food consumption, age, BMI, smoking status, alcohol use, lymphocyte percentage, and white blood cell count. Values of histone modifications was natural logarithmic transformed, and DNA methylation levels were logit transformed.