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. 2020 Jul 21;19(11):4690–4697. doi: 10.1021/acs.jproteome.0c00392

Figure 3.

Figure 3

Incorporation of 2′-F,Me-UTP, 3′-F-dTTP, TFV-DP, and 3′-N3-dTTP by SARS-CoV-2 RdRp to terminate the polymerase reaction. The sequences of the primer and template used for these extension reactions, which are at the 3′ end of the SARS-CoV-2 genome, are shown at the top of the figure. Polymerase extension reactions were performed by incubating (a) 2′-F,Me-UTP, (b) 3′-F-dTTP, (c) UTP + TFV-DP, and (d) 3′-N3-dTTP with preassembled SARS-CoV-2 polymerase (nsp12, nsp7, and nsp8), the indicated RNA template and primer, and the appropriate reaction buffer, followed by detection of reaction products by MALDI-TOF MS. The accuracy for m/z determination is ±10 Da.