TABLE 1.

Oligonucleotide primers used for PCR in this study.

Primers	Oligo-nucleotides (5′-3′)	Restriction sites
ORF711-F	GAATTCCATATGCGTTCCCG GCCTGGCAG	NdeI
ORF711-R	TCCGCTCGAGCTGCAGGGTA GCGGCCG	XhoI
ORF3245-F	GAATTCCATATGATGAGCCG TCCCGCCGGATC	NdeI
ORF3245-R	TCCGCTCGAGGGCGCCCACC TGCACCG-3′	XhoI
ORF3636-F	GAATTCCATATGGCCCATGC CGCACCGGCATG	NdeI
ORF3636-R	CCCAAGCTTGTACTTCAACG CGCTGCAG	HindIII
ORF3638-F	GAATTCCATATGGCTGAATG GGCGGAAGGCAAC	NdeI
ORF3638-R	TCCGCTCGAGGTGTGATGTC CGGCGTACGC	XhoI
ORF3639-F	GAATTCCATATGCGTACCCG GTATGGCAGG	NdeI
ORF3639-R	TCCGCTCGAGCATGGGCAAC ATGAACAAG	XhoI
ORF3769-F	GAATTCCATATACCGAGATC GCCCCGTACTTC	NdeI
ORF3769-R	TCCGCTCGAGGGTGCTGGGC AACGGCCGC	XhoI

The underlined sequences are restriction enzyme cutting sites.