TABLE 5.
Enzyme activities of recombinant key chitinolytic enzymes toward chitinous substrates.
| Enzymes |
Enzyme activity (U/mg of protein) |
||||
| CC | (GlcNAc)2 | pNP-GlcNAc | pNP-(GlcNAc)2 | pNP-(GlcNAc)3 | |
| Cm711 | 3.6 ± 0.2 | nda | nd | 2.3 ± 0.1 | 1.7 ± 0.1 |
| Cm3245 | 0.02 ± 0.001 | 4,233 ± 210 | 3,902.9 ± 130 | 2,140 ± 160 | 1,082 ± 62 |
| Cm3636 | 2.1 ± 0.1 | nd | nd | 6.2 ± 0.3 | 0.7 ± 0.02 |
| Cm3638 | 4.6 ± 0.2 | nd | nd | 8.5 ± 0.7 | 1.6 ± 0.1 |
| Cm3639 | 15.3 ± 0.3 | 0.5 ± 0.02 | 0.2 ± 0.01 | 27.3 ± 0.6 | nd |
| Cm3769 | 4.1 ± 0.4 | 13.5 ± 0.9 | 10.3 ± 0.6 | 13.3 ± 1.2 | 3.2 ± 0.2 |
aActivity was not detected. Reaction mixture (1 mL) containing enzyme and 10 g/L CC, (GlcNAc)2, or 0.5 mM pNP-(GlcNAc)1–3 were incubated at 37°C and pH 7.0.