KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-GFP (clone JL-8) antibody | Clontech | Cat# 632380; RRID: AB_10013427 |
| Rabbit polyclonal anti-Actin antibody | Sigma-Aldrich | Cat# A2066; RRID: AB_476693 |
| Anti-Puromycin Antibody, clone 12D10 | Millipore | Cat# MABE343 RRID: AB_2566826 |
| Anti mouse IgG, HRP linked Antibody | Cell Signaling Technology | Cat#7076S RRID: AB_330924 |
| Anti rabbit IgG, HRP linked Antibody | Cell Signaling Technology | Cat#7074S RRID: AB_2099233 |
| Bacterial and Virus Strains | ||
| C. elegans ORF RNAi library Collection | Dharmacon | Car# RCE1181 |
| OP50 E coli Strain | Caenorhabditis Genetics Center | OP50 |
| Drechmeria coniospora | J. Ewbank Lab | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| C. elegans CED-3 | Weaver et al., 2014 | N/A |
| TRIzol® Reagent | Thermo Fisher Scientific | Cat#15596026 |
| EasyTag™ L-[35S]-Methionine | PerkinElmer | Cat# NEG709A500UC |
| Critical Commercial Assays | ||
| ABsolute Blue qPCR SYBR Green Low ROX Mix | Thermo Fisher Scientific | Cat# AB-4322/B |
| TruSeq Ribo Profile (Mammalian) Library Prep Kit | Illumina | Cat# RPHMR12126 |
| Ribo-Zero Gold rRNA Removal Kit (H/M/R) | Illumina | Cat# MRZG12324 |
| Pierce MS-Compatible Magnetic IP Kit (Protein A/G) | Thermo Scientific | Cat# 90409 |
| SuperScript™ III Reverse Transcriptase | Thermo Fisher Scientific | Cat# 18080044 |
| TnT® Quick Coupled Transcription/Translation System | Promega | Cat# L1170 |
| Deposited Data | ||
| RNA-seq and ribosome profiling data | GEO Database | GSE145983 |
| Experimental Models: Organisms/Strains | ||
| C. elegans: Strain MT1522:ced-3(n717) | Caenorhabditis Genetics Center | WB Strain: MT1522 |
| C. elegans: Strain KU25:pmk-1(km25) | Caenorhabditis Genetics Center | WB Strain: KU25 |
| C. elegans: Strain MT3002:ced-3(n1286) | Caenorhabditis Genetics Center | WB Strain: MT3002 |
| C. elegans: Strain RB2071:ced-3(ok2734) | Caenorhabditis Genetics Center | WB Strain: RB2071 |
| C. elegans: Strain MT2547:ced-4(n1162) | Caenorhabditis Genetics Center | WB Strain: MT2547 |
| C. elegans: Strain MT6347:ced-3(n2433) | Caenorhabditis Genetics Center | WB Strain: MT6347 |
| C. elegans: Strain KU4:sek-1(km4) | Caenorhabditis Genetics Center | WB Strain: KU4 |
| C. elegans: Strain AU1:sek-1(ag1) | Caenorhabditis Genetics Center | WB Strain: AU1 |
| C. elegans: Strain AU3:nsy-1(ag3) | Caenorhabditis Genetics Center | WB Strain: AU3 |
| C. elegans: Strain MH5935:pmk-1(km25) ced-3(n717) | This Study | MH5935 |
| C. elegans: Strain MH5936:ced-3(n717);frIs7[nlp-29p::gfp] | This Study | MH5936 |
| C. elegans: Strain IG274 frIs7 [nlp-29p::GFP + col-12p::DsRed] | Caenorhabditis Genetics Center | WB Strain: IG274 |
| C. elegans: Strain BPW47 ced-3(n717);vpIs1 [lin-15(+) + elt-3::GFP] | This Study | BPW47 |
| C. elegans: Strain JG5 vpIs1 [lin-15(+) + elt-3::GFP] | Caenorhabditis Genetics Center | WB Strain: JG5 |
| C. elegans: Strain BPW24 pmk-1(miy7 [pmk-1(D327E)]) frIs7 [nlp-29p::GFP + col-12p::DsRed] IV | This Study | BPW24 |
| C. elegans: Strain:BPW45 ced-1(e1735);pmk-1(miy7[pmk-1 (D327E)]) | This Study | BPW45 |
| C. elegans: Strain: BPW47 ced-1(e1735) | This Study | BPW47 |
| C. elegans: Strain: MT5811 ced-1(e1735) I; ced-3(n717) IV | Caenorhabditis Genetics Center | WB Strain: MT5811 |
| C. elegans: Strain:BPW3 ced-3(n2427) IV; nIs106 [lin-11::GFP + lin-15(+)] X | This Study | BPW3 |
| C. elegans: Strain:BPW49 nIs106 [lin-11::GFP + lin-15(+)] X | This Study | BPW49 |
| C. elegans: Strain: BPW50 ced-3(n717)IV; nIs106 [lin-11::GFP + lin-15(+)] X | This Study | BPW50 |
| C. elegans: Strain: BPW51 pmk-1(miy7 [pmk-1 (D327E)])IV; nIs106 [lin-11::GFP + lin-15(+)]X | This Study | BPW51 |
| C. elegans: Strain: SJ4005 zcIs4 [hsp-4::GFP] V. | Caenorhabditis Genetics Center | WB Strain: SJ4005 |
| C. elegans: Strain: AU78 agIs219 [T24B8.5p::GFP::unc-54-3′ UTR + ttx-3p::GFP::unc-54-3′ UTR] III | Caenorhabditis Genetics Center | WB Strain: AU78 |
| C. elegans: Strain: BPW18 ced-3(n2433);frIs7 [nlp-29p::GFP + col-12p::DsRed] IV | This Study | BPW18 |
| Oligonucleotides | ||
| Primers 1-12 | This Study | See Table S3 |
| Recombinant DNA | ||
| pDD162_C743 | This Study | dpy-10 sgRNA |
| pDD162_C768 | This Study | pmk-1 sgRNA |
| pWE592 | This Study | pTNT_wt pmk-1 |
| pWE593 | This Study | pTNT_pmk-1 D99A |
| pWE594 | This Study | pTNT_pmk-1 D112A |
| pWE595 | This Study | pTNT_pmk-1 D241A |
| pWE596 | This Study | pTNT_pmk-1 D327A |
| pWE597 | This Study | pTNT_pmk-1 D335A |
| pWE598 | This Study | pTNT_pmk-1 D338A |
| pWE599 | This Study | pTNT_pmk-1 D346A |
| pWE601 | This Study | pTNT_pmk-1 D327E |
| Software and Algorithms | ||
| Fiji (ImageJ) | http://fiji.sc | Ver 1.50i; RRID: SCR_002285 |
| Graphpad Prism | http://www.graphpad.com/ | Ver 6.07; RRID: SCR_002798 |
| WormBase | RRID: SCR_003098 | |
| OMIM | http://www.omim.org | RRID: SCR_006437 |
| UniProt | http://www.uniprot.org/ | RRID: SCR_002380 |
| SeqPurge | https://github.com/imgag/ngs-bits | N/A |
| Bowtie 2 | http://bowtie-bio.sourceforge.net/bowtie2/index.shtml | RRID: SCR_005476 |
| STAR | https://github.com/alexdobin/STAR | RRID: SCR_015899 |
| HTSeq | https://htseq.readthedocs.io/en/release_0.10.0/ | RRID: SCR_005514 |
| edgeR | http://bioconductor.org/packages/release/bioc/html/edgeR.html | RRID: SCR_012802 |
| WormBase Enrichment Analysis |
https://wormbase.org/tools/enrichment/tea/tea.cgi |
N/A |