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. Author manuscript; available in PMC: 2020 Nov 4.
Published in final edited form as: Nat Struct Mol Biol. 2020 Feb 3;27(2):160–167. doi: 10.1038/s41594-019-0371-2

Extended Data Fig. 8. HxxxW motif rotamers and comparison of the closed BM2 structures in lipid bilayers versus detergent micelles.

Extended Data Fig. 8

(a) Structural ensembles of H19 and W23 in the conserved HxxxW conduction motif at high pH (left) and low pH (right). The H19 χ1 is trans but the χ2 is not constrained well by experimental data. W23 predominantly adopts the t90 rotamer in both closed and open structural ensembles. (b-c) Comparison of the high-pH BM2 TM structure in lipid bilayers versus in detergent micelles. (b) Solid-state NMR structure determined here in POPE membranes. (c) Solution NMR structure determined in DHPC micelles 1.