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. Author manuscript; available in PMC: 2020 Nov 4.
Published in final edited form as: Nat Struct Mol Biol. 2020 Feb 3;27(2):160–167. doi: 10.1038/s41594-019-0371-2

Extended Data Fig. 10. Pulse sequences of key 2D and 3D correlation experiments used for determining the structures of closed and open BM2 channels.

Extended Data Fig. 10

(a) 3D CCC experiment. The first 13C spin diffusion period is short to obtain intra-residue correlations while the second is long to obtain inter-residue cross peaks. (b) Water-edited 2D CC experiment. A selective 90˚ pulse excites the water 1H magnetization, a 1H T2 filter removes the rigid protein magnetization, then the water magnetization is transferred to the protein. Filled and open rectangles indicate 90° and 180° pulses, respectively. (c) 3D NCC experiment involving an out-and-back 15N-13C TEDOR period followed by 13C spin diffusion. The experiment simultaneously detects NCACX and NCOCX correlations. (d) Frequency-selective 13C-19F REDOR for distance measurements. (e) 3D NC-resolved N-H dipolar-doubled DIPSHIFT experiment for measuring helix orientations.