Figure 4.
(A) Competition experiments show that 8 selectively photo-crosslinks PTPσ-Fc in vitro (n = 3). (B) Selective photo-crosslinking of endogenous PTPσ and EphA4 with 8 in cortical neurons (n = 4). (C) TMT ratios of 8-treated versus vehicle (Veh)-treated neurons showing proteins enriched by ⩾5-fold (blue). (D) TMT ratio plot of the 54 membrane-associated and ECM proteins (blue). (E) Functions of the identified CS-E interactors. (F) Validation by ELISA of new CS-E-binding proteins (n = 2). Data represent the mean ± SEM. ****P ≤ 0.0001, ***P ≤ 0.001, **P ≤ 0.01.