Table 1.
Nanoconjugate Nomenclature, Formula, ζ Potential, Calculated Molecular Mass, and SEC-HPLC Retention Time
| nanoconjugate | components | ζ potential (mV) |
grouped by ζ potential |
molecular mass (g/mol) |
SEC-HPLC retention time (rt, min) |
|---|---|---|---|---|---|
| P (nanoconjugate backbone) | PMLA | −22.8 | Group 1 | 50,000 | 7.52 |
| P/rh | PMLA/rh(1%) | −22.9 | 56,000 | 7.23 | |
| P/LLL/rh | PMLA/LLL(40%)/rh(1%) | −16.5 | Group 2 | 11,4000 | 7.10 |
| P/cTfRL/rh | PMLA/cTfRL(2%)/rh(1%) | −15.2 | 81,000 | 7.05 | |
| P/M4/rh | PMLA/M4(2%)/rh(l%) | −14.6 | 80,000 | 7.10 | |
| P/AP2/rh | PMLA/AP2(2%)/rh(1%) | − 11.5 | 106,000 | 7.18 | |
| P/LLL/AP2/rha | PMLA/LLL(40%)/AP2(2%)/rh(1%) | − 11.6 | Group 3 | 164000 | 7.215 |
| P/LLL/M4/rhc | PMLA/LLL(40%)/M4(2%)/rh(1%) | −10.4 | 138,000 | 7.20 | |
| P/LLL/cTfRL/rhd | PMLA/LLL(40%)/cTfRL(2%)/rh(1%) | −9.58 | 139,000 | 7.22 | |
| P/LLL/AP2/D1/rhf | PMLA/LLL(40%)/AP2(2%)/D1(2%)/ rh(1%) |
−7.44 | Group 4 | 207,000 | 7.05 |
| P/LLL/B6/rhe | PMLA/LLL(40%)/B6(2%)/rh(1%) | −6.1 | 153,000 | 7.5 | |
| P/LLL/AP2/M4/rh | PMLA/LLL(40%)/AP2(2%)/M4(2%)/ rh(1%) |
−5.5 | 188,000 | 7.05 | |
| P/LLL/AP7/rhb | PMLA/LLL(40%)/AP7(2%)/rh(1%) | −5.48 | Group 5 | 165,000 | 7.27 |
| P/LLL/AP2(4%)/rh | PMLA/LLL(40%)/AP2(4%)/rh(1%) | −2.2 | 217,000 | 7.47 |
a
Peptide sequence: TFFYGGSRGKRNNFKTEEYCNH2.
b
Peptide sequence: TFFYGGSRGRRNNFRTEEYCNH2.
c
Peptide sequence: H-[Dap] KAPETAL D-NH2, monocyclic lactam bridged.
d
Peptide sequence: CRTIGPSVC-NH2, monocyclic disulfide bridged.
e
Peptide sequence: CGHKAKGPRK.
f
Peptide sequence: qshyrhispaqvc.