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. 2020 Nov 3;10:18902. doi: 10.1038/s41598-020-75732-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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BACH2-mediated repression of luciferase expression is inhibited by the HDAC3 inhibitor molecule RGFP966. (a, b), Luciferase activity in inducible-BACH2 reporter line after 6 h stimulation with PMA/ionomycin and pre-treatment with or without 1 μg/ml tetracycline and with 12.5 μM of RGFP966 or without (indicated as Veh). (c) Western blot analysis of the abundance of BACH2 within total protein lysates from cells in (a, b) treated with or without 12.5 µM RGFP966. Unpaired two-tailed Student’s t test (a, b). (a, b) Data are representative of 2 independently repeated experiments with 3 culture replicates per condition. Bars and error represent mean (SD); ns not significant; ****P < 0.0001.