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. 2020 Nov 3;10:18899. doi: 10.1038/s41598-020-76043-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Spike-in assay using SARS-CoV-2-positive and -negative nasopharyngeal swabs from patients. (A) Scheme of preparing spike-in solutions. SARS-CoV-2-positive samples with high, intermediate and low viral copies were used. These different viral loads were diluted with 4, 9 and 19 SARS-CoV-2-negative samples. The final solution was made at × 5, × 10 and × 20 dilutions and used for RT-qPCR analysis. (B) RT-qPCR analysis determined the copy numbers in the spike-in solutions. Each of the three patients had a different viral load corresponding to low, intermediate, and high. These SARS-CoV-2 positive samples were diluted with negative samples. The assay was used with NIID-N1 (pink) and NIID-N2 (blue). Bar plot shows the copy number (log₁₀ copies/μL) in original (× 0) and diluted (× 5, × 10 and × 20) samples. All data represent the mean ± SD.