Table 2.

Identity, sources, and working dilution of antibodies used in immunohistochemical studies.

Target	Primary antibody supplier	Origin (catalog no)	Dilution	incubation	Antigen retrieval	secondary antibody-incubation time
CD34	Rat anti-mouse CD34 antibody ( e bioscience, San Diego, CA	Mouse CD34 monoclonal antibody (clone: RAM34) (Cat.no 14–0341-85)	1:100	Over night	Boiling in citrate buffer (pH 6.0), 20 min Goat	Goat anti-mouse IgG (H + L) secondary antibody Catalog # 31569 Dilution ; 1:100 One hour at room temperature
CD117	Anti-CD117 (e Bioscience, San Diego, CA)	Mouse Cd117 monoclonal antibody (clone. ACK2) (Cat. no. 14–1172–82)	1:100	Over night	Boiling in citrate buffer (pH 6.0), 20 min
VEGF	Anti-VEGF rabbit monoclonal antibody boster biological technology	Rabbit Monoclonal (clone:ADB-22) CA 94,566	1:100	Over night	Boiling in citrate buffer (pH 6.0), 20 min	Goat anti-rabbit secondary antibody (cat. no. K4003, EN Vision + TM System Horseradish Peroxidase Labelled Polymer; Dako) Ready to use 30 min at room temperature
MPP-9	Anti-MPP9 Thermo Fischer Scientific, Lab vision Corporation, Fremont, USA	Mouse (mc, Ab-1) Clone D(33)376 RB-9423-PO Rabbit polyclonal	1:30	Over night	Boiling in citrate buffer (pH 6.0), 20 min	Biotinylated goat Anti-Polyvalent, Anti-mouse Igg + Anti-Rabbit Igg, Thermo Fisher Scientific, The UK. Lab Vision Corporation; USA Ready to use One hour at room temperature

Antibodies used that showed reactivity in fish species.