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. 2020 Nov 3;10:18932. doi: 10.1038/s41598-020-75988-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Genomic comparison of recombination regions involving capsule locus among donor, transformant, and recipient. For both recombination events (a, b), encapsulated serotype 3 S. pneumoniae strain WU2 served as the donor strain. (a) NESp strain MNZ41 is the recipient and 41S3 is the transformant. (b) NESp strain MNZ67 is the recipient and 67S1 is the transformant. Trans = Transformant, Recip = Recipient. Arrows indicate direction of open reading frame (ORF). Blue arrows indicate either dexB or aliA capsule-flanking genes to demarcate the capsule region. Magenta lines represent contig breakpoints. Black pointers represent recombination breakpoints. Grayscale represents % nucleotide identity between sequences.