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. 2020 Nov 3;10:18924. doi: 10.1038/s41598-020-76033-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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CRISPR/Cas9-mediated generation of a POLD1-knockout model. (A) Schematic overview of the targeting procedure, displaying CRISPR/Cas9-mediated integration of the repair template in exon 2a of the POLD1 locus. (B) PCR detecting the POLD1-KO and –WT alleles as well as (C) the Cre/loxP-mediated excision of the puromycin resistance cassette. Dashed lines indicate cropping, and the original gels are displayed in Figure S1.