Table 5.

RNA-seq result validation by qPCR.

Putative function	Gene	RNA-seq	qPCR
		Expression in TR+ teeth	Expression in TR+	Fold change normalised to TR-	p value
Osteoclast differentiation	SPI1	↑**	↑**	2.60	0.024
	RANKL	↑	↑	1.35	0.279
	OPG	↓**	↓**	0.44	0.037
	RANKL/OPG	–	↑**	3.10	0.036
Osteoclast genes	MMP9	↑**	↑**	4.52	0.016
	ACP5	↑**	↑**	2.95	0.019
	CTSK	↑*	↑**	2.81	0.003
Calcium signalling in osteoclast	P2X2R	↑**	↑**	3.04	0.025
	P2X4R	↑**	↑**	2.28	0.039
	P2X6R	↑**	↑*	2.81	0.079
	PCLB4	↑**	↑	1.83	0.100
Common genes in TR + teeth (pH homeostasis)	CA4	↑**	↑*	2.67	0.052
	CA6	↑**	↑**	5.51	0.040

Total n = 6 from TR –ve = 3, from TR + ve = 3, *; 0.05 < p < 0.1, **; p < 0.05 by student t-test.