Materials
| Name of Reagent/ Equipment | Company | Catalog Number | Comments/Description |
|---|---|---|---|
| PBS | Gibco | 10-010-023 | |
| Pen Strep | MP Biomedicals | 91670049 | |
| Bovine Collagen Solution | Advance Biomatrix | 5005 | |
| Pig eyes with lids attached | Pel-freeze, Arkansas | N/A | |
| 6.0 mm trephine | Katena | K28014 | |
| Surgical Blade | Personna | 0.009 | |
| Small scissor | Fisher | 895110 | |
| Forceps | Fisher | 08953-F | |
| Kim Wipes | Kimberly-Clark™ 34120 | 06–666 | |
| 60 mm cell culture dishes | Falcon | 08–772B | |
| DMEM/F-12 | Gibco | 11330 | |
| ITS Liquid Media Supplement | Sigma | I3146 | |
| RPMI 1640 Vitamins Solution | Sigma | R7256 | |
| Glutathione | Sigma | G6013 | |
| 1% L-glutamine solution | Gibco | 25030–081 | |
| MEM Non-essential amino acids solution | Gibco | 11140 | |
| 1mM Sodium pyruvate solution | Gibco | 11360 | |
| 1% ABAM | Sigma | A7292 | |
| 0.5% Gentamicin | Sigma | 30-005-CR | |
| 1uM Vitamin C | Wako | 070–0483 | 2-0-aD Glucopyranosyl-Ascorbic Acid |
| 10% Iodine | Fisher Chemical | SI86-1 | |
| Tissue Path Cassettes | Fisher | 22–272416 | |
| Normal Goat Serum | Jackson Immuno Research | 005-000-121 | |
| Mounting Media | Thermo Scientific | TA-030-FM | |
| Safe Clear | Fisher | 314–629 | |
| Ethyl Alcohol | Ultra Pure | 200CSGP | 200 Proof, diluted at 100%, 70%, 50%) |
| Sodium citrate | Fisher | BP327 | 10mM, pH 6.4 |
| Hematoxylin | EMD Millipore | M10742500 | |
| Bluing agent | Ricca Chemical Company | 220–106 | |
| 1% Triton X-100 | Fisher | 9002-93-1 | Diluted in PBS |
| 0.1% Tween 20 | Fisher | BP337 | Diluted in PBS |
| 3% Hydrogen Peroxide | Fisher | H324 | |
| DAB Kit | Vector Laboratories | SK-4100 |