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. 2020 Nov 3;3:643. doi: 10.1038/s42003-020-01374-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Schematic showing a rapid 3-step protocol to exchange mCherry in VACV-mCh for venus, including the steps taken to track selection. b Proportions of mCherry-positive (mCh⁺), double-negative (DN), double-positive (DP) and Venus-positive viruses (Venus⁺) following homologous recombination and Cas9 selection are shown. One hundred plaques were counted per condition. Pie charts and adjacent numbers are means across two independent experiments.