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. 2020 Nov 3;11:5552. doi: 10.1038/s41467-020-19370-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic of ribosome complexes programmed with mRNAs that extend 0-21 nucleotides beyond the P site (AUG codon). b Time courses of ArfB-catalyzed [³⁵S]-fMet release from 70S complexes with mRNAs with or without poly-adenosine overhangs. CPM readout was normalized relative to the signal for the mRNA with no overhang (AUG) at 1 h (N = 3 independent experiments). c Apparent rates (v₀) of ArfB-catalyzed fMet release from ribosomes with mRNAs containing poly-pyrimidine (denoted Y), poly-A, and poly-arginine/leucine-encoding sequences (codons listed) of different lengths (N.D. not detected). (Error bars represent errors of fit from N = 3 independent experiments).