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. 2020 Apr 23;48(14):7864–7882. doi: 10.1093/nar/gkaa277

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Identified over- and underexpressed proteins and their related signalling pathways from NCH82 glioma cell nucleus fraction following the Forskolin treatment time course (16, 24 and 48 h) compared to the control condition. (A) Heat maps of the LFQ variation for 16 h (1), 24 h (2) and 48 h (3) treatment with Forskolin, each compared to the control condition, generated after raw nLC–MS/MS data interrogated by MaxQuant with LFQ and further processed in Perseus using a t-test showing the proteins over- and underexpressed. (B) GO terms associated with overexpressed proteins (in A) under Forskolin treatment time course. The untreated condition presents the mRNA processing regulation underexpression that was observed for the corresponding stimulation times. After 16 h Forskolin, an overexpression of proteins in specific pathways such as the initiation of the translation and the nucleotide metabolic process appears. At 24 h, two specific enrichments were found related to collagen formation and cytoskeleton. At 48 h, no specific enrichment was observed. All the other cellular processes are shared between all Forskolin treatment times (time independent).