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REMSA measurement of PCBP2 construct affinity for SLIVm. Biotinylated SLIVm was tracked in native PAGE after incubation with increasing amounts of PCBP2-FL (LHS) or PCBP2-ΔKH3 (RHS) to compare their binding affinities. KD,app values were determined as the protein concentration to effect half maximal binding to the RNA. A higher affinity of KD,app = 0.25 μM was observed for PCBP2 and a lower affinity of KD,app = 2.3 μM was observed for PCBP2-ΔKH3.
