Figure 1.

Capillary electrophoresis measurements of ligation efficiency. (A) Schematic of the CE assay. A FAM labeled degenerate RNA oligo was used as a substrate for sequential 3′ and 5′ ligations using either the single-stranded or randomized splint methods. (B) Example CE traces showing single-stranded (blue) and randomized splint (red) reactions. The major products and side products are labeled with letters according to the key below the traces. (C) Quantification of the main ligation products as a percentage of the total amount of FAM labeled oligo. Experiments were performed in quadruplicate.