Figure 2.

Inhibition of CDK12^as affects transcription elongation. (A) Experimental design. mNET-seq using an antibody against total pol II was performed in CDK12^as HEK293 cells after treatment with 15 min of DMSO solvent control or 7.5 μM NM. (B) Metagene analysis comparing the average mNET-seq signal before and after CDK12^as inhibition for 15 min of expressed genes. (C) Violin plots of the different length classes noted show the ratio of mNET-seq signal after 15 min inhibition of CDK12^as compared to control. (D) Metagene analysis for the different length classes noted comparing the average mNET-seq signal before and after CDK12^as inhibition for 15 min. The mNET-seq reads were averaged and aligned at their TSSs and (pA)-sites.