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. 2020 Jun 17;48(14):7712–7727. doi: 10.1093/nar/gkaa514

Figure 5.

Figure 5.

CDK12 activity is required for stable association of elongation factors with chromatin. (A) Meta-analysis of LEO1 subunit of the PAF1 complex (PAF1C) ChIP-seq across scaled expressed genes. (B) Meta-analysis of SPT6 ChIP-seq across scaled expressed genes. (C) Meta-analyses of LEO1 ChIP-seq ratioed to the total pol II signal before and after CDK12as inhibition for 15 min across scaled expressed genes for the different length classes noted. (D) Meta-analyses of SPT6 ChIP-seq ratioed to the total pol II signal before and after CDK12as inhibition for 15 min across scaled expressed genes for the different length classes noted. (E) Western blots of CDK12as cell chromatin extracts. Cells are either treated with 7.5 μM 1-NM-PP1 or DMSO for 15 and 30 min as noted. The antibodies used are indicated on the right. Histone H3 was used as a loading control. (F) Quantitation of chromatin protein levels of SPT6, LEO1, CDC73, and histone H3 in CDK12as cells relative to DMSO controls. Error bars = s.e.m. (n = 3 biological replicates). Statistical test: two-tailed unpaired t test, ns = not significant, * P < 0.05, *** P < 0.001.