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. 2020 Jun 25;48(14):7786–7800. doi: 10.1093/nar/gkaa533

Figure 2.

Figure 2.

Monitoring the growth of Zobellia galactanivorans on alginate. (A) Growth at 20°C, 180 rpm in minimum medium supplemented with either maltose or alginate FD176 as a sole carbon source (mean ± s.e.m., n = 4). (B, C, D) Analysis of soluble degradation products measured in spent medium of Z. galactanivorans grown in MMM supplemented with alginate FD176. (B) Concentration of soluble sugar reducing ends (expressed as glucose equivalent) and unsaturated uronic residues (mean ± s.e.m., n = 4). (C) MALLS analysis of degradation products. Bars denote the mean concentration (left axis) of products from different size fractions (from 0 to 200 kDa). Black circles denote the remaining percentage of total products detected compared to T0 (right axis, mean ± s.e.m., n = 4). (D) Representative HPAEC analysis of degradation products after 30.5 hours of cultivation, as compared to standards enriched in alginate tri-, tetra, penta- and hexa-saccharides.