Table 1.

Sequences of 10 nt 2CNqA-ssDNAs and melting temperature (T_m) of their duplexes and their unmodified duplex counterparts^a

Sequence namea	5′-3′ Sequence (X = 2CNqA)	2CNqA-dsDNA Tm [°C]	Unmodified dsDNA Tm [°C]	ΔTm [°C]
AA	CGC AAX ATC G	42.4 ± 0.3	43.3	−0.9
AC	CGC AAX CTC G	48.8 ± 0.3	47.0	1.8
AG	CGC AAX GTC G	45.5 ± 0.2	45.8	−0.3
AT	CGC AAX TTC G	46.1 ± 0.1	43.5	2.6
CA	CGC ACX ATC G	52.1 ± 0.2	46.9	5.2
CC	CGC ACX CTC G	58.1 ± 0.3	50.5	7.6
CG	CGC ACX GTC G	55.8 ± 0.1	49.5	6.3
CT	CGC ACX TTC G	56.0 ± 0.3	47.0	9.0
GA	CGC AGX ATC G	45.0 ± 0.1	45.3	−0.3
GC	CGC AGX CTC G	51.6 ± 0.3	49.0	2.6
GG	CGC AGX GTC G	49.1b	48.0	1.1
GT	CGC AGX TTC G	48.7b	45.6	3.1
TA	CGC ATX ATC G	44.4 ± 0.3	41.1	3.3
TC	CGC ATX CTC G	49.5 ± 0.3	43.5	6.0
TG	CGC ATX GTC G	47.4b	43.5	3.9
TT	CGC ATX TTC G	47.4 ± 0.4	40.5	6.9

^aSequence names indicate bases surrounding 2CNqA on the 5′ and 3′ side. Measured in 13 mM sodium phosphate buffer, 100 mM NaCl, pH 7.5.

^b Measured only once.