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. 2020 Jun 19;48(14):7640–7652. doi: 10.1093/nar/gkaa525

Table 4.

The melting temperature (Tm) of a 25 bp unmodified sequence (RNA-D0:RNA-A0) and the corresponding 2CNqA-dsRNA (RNA-D13:RNA-A0), tCnitro-dsRNA (RNA-D0:RNA-A10) and doubly-labelled dsRNA (RNA-D13:RNA-A10)a

dsRNA T m [°C] ΔTm [°C]
RNA-D0:RNA-A0 67.0 ± 0.1 –
RNA-D13:RNA-A10 68.7 ± 0.2 1.7
RNA-D13:RNA-A0 68.5 ± 0.2 1.5
RNA-D0:RNA-A10 68.2 ± 0.3 1.2

aMeasured in 13 mM sodium phosphate buffer, 100 mM NaCl, 1.0 mM EDTA, pH 7.4.